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CHIR99021 inhibits the growth of Neuro-2a cells. (A–C) Western blot analysis and quantification of the expression of cyclin D1 and Ki67 protein. The relative expression levels are normalized to β-actin and then expressed as the fold of control group. (D) Detection of cell proliferation by electric cell-substrate impedance sensing. The data are expressed as the mean ± SEM. * p < 0.05.
