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. 2023 Feb 9;12(2):423. doi: 10.3390/antiox12020423

Figure 3.

Figure 3

The effect of H2Sn and S8 on the PerR-repressed reporter. (A) Schematic representation of the test plasmid (pBBR-perR-PprxI-egfp). The expression of egfp was initiated by the prxI promoter (PprxI), and the interaction between PerR and PprxI, which was associated with the inducers that affected GFP fluorescence. H2Sn (A) and S8 (B) induction increased the intensity of GFP fluorescence in E. coli BL21 (pBBR-perR-PprxI-egfp). (C) The mutation of Cys affected the function of PerR in E. coli BL21 (pBBR-perR-PprxI-egfp). C19S, C121S, C124S, C137S, C161S, and C163S represented single mutations of Cys to Ser in PerR. (D) The mutation of His did not affect the function of PerR in E. coli BL21 (pBBR-perR-PprxI-egfp). H13A, H62A, H116A, H117A and H118A represented single mutations of His to Ala in PerR. FI/OD represents the fluorescence intensity of per OD cells. All data are averages from three samples with standard deviations (error bars). The experiment was repeated at least three times. *, p < 0.1; **, p < 0.01; ****, p < 0.0001; ns, not significant (paired t test).