TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| S. meliloti | ||
| RCR2011 | SU47 (wild-type) | 42 |
| Rm5000 | SU47 rif-5 | 10 |
| Rm1021 | SU47 str-21 | 30 |
| UNA186 | Rm5000, frcC::TnphoA | This work |
| E. coli | ||
| DH5α | F−endA1 hsdR17 supE44 thi-1 recA1 gyrA96 relA1Δ(argF-lacZYA) | Bethesda Research Laboratories |
| XL2Blue | recA1 endA1 gyrA96 thi-1 hsd17 supE44 relA1 lac (F′ proAB lacIqZΔM15 Tn10(Tetr) Amy Camr) | Stratagene |
| S17.1 | E. coli 294, with RP4 | 48 |
| A. tumefaciens | ||
| GM19023 | C58 cured of pAtC58 and pTiC58 | 42 |
| At125 | GM19023 pRmeSu47b | 11 |
| At128 | GM19023 pRmeSu47a | 11 |
| Plasmids | ||
| pBluescript SK II(−) | Derivative of pUC19 with f1(−), oriR, Ampr | Stratagene |
| pLAFR1 | IncP cosmid cloning vector, Tetr | 14 |
| pMTnphoA2 | pSUP102::TnphoA, Tetr Ampr Neor | 32 |
| pMR20 | RK2-based cloning vector, Tetr | 21 |
| pBSFru | pBluescript, 14-kb Stul-EcoRI fragment with frc genes of S. meliloti | This work |
| pMRFru | pMR20, 4.4-kb KpnI-NruI fragment with frcBCAK genes of S. meliloti | This work |
| pMRF2 | pMR20, 4.4-kb HindIII-NruI fragment with frcBCAK genes of S. meliloti | This work |