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. 2001 Aug;183(16):4718–4726. doi: 10.1128/JB.183.16.4718-4726.2001

FIG. 1.

FIG. 1

Generation of a nonpolar insertion mutation. IDM results in a single-crossover recombination event, insertion of the plasmid into the chromosome, and duplication of homologous sequences. The strong ermC promoter drives the transcription of downstream genes.