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. 2001 Aug;183(16):4718–4726. doi: 10.1128/JB.183.16.4718-4726.2001

TABLE 2.

PCR primers

Primer name Sequence
pMOB-polF 5′-GTA AAA CGA CGG CCA GTG AGC GCG-3′
pMOB-polR 5′-AAC AGC TAT GAC CAT GAT TAC GCC AAG-3′
pORI-F 5′-GAA GCT AGC TTA TGC GGT GTG AAA TAC-3′
pGCU-R 5′-AAC GTT GTT GCC ATT GCT GC-3′
27F 5′-CGG TTG ACA ACG CGG ATA TTT CAG G-3′
TraH2R 5′-GCC AGG TCA AAG GCT ACA CC-3′
23F 5′-GAT TCG TTC TGC TGC TGA GGC C-3′
TraH-up 5′-CGA ATG CAT CTG CTA TAA CCG CTT CAT GG-3′
TraH-down 5′-CGA GAT ATC GTA CAG CTT TCT CCG TTA CC-3′