Skip to main content
. 2023 Jan 18;12(2):200. doi: 10.3390/antibiotics12020200

Figure 1.

Figure 1

In vitro evolution assays for L-Ara4N deficient mutants of P. aeruginosa PAO1 and PA14 (ΔarnBCA) carrying plasmids for the ectopic expression of EptA (pMEeptA) or MCR-1 (pMEmcr-1), or the empty plasmid pME6032 as the control. Strains were evolved through serial passages in the presence of increasing concentrations of colistin (up to 64 μg/mL). Five biological replicates (independent cultures) were analyzed for each strain. The graphs show a representative curve for each strain, while the curves of the other replicates are provided in Figure S1. The “×” symbol highlights cultures that went extinct during the evolution experiment.