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. 2023 Jan 18;12(2):200. doi: 10.3390/antibiotics12020200

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Overview of the results of the transposon mutagenesis screening. (a) Number of transposon mutants of PAO1 Parn::lux or PAO1 PeptA::lux showing higher luminescence emission, both on agar plates and during planktonic growth, and/or higher colistin MIC compared with the corresponding parental strain. (b) Correlation between PeptA or Parn activity (x axis) and colistin MIC (y axis) for the selected transposon mutants of PAO1 Parn::lux (circles) or PAO1 PeptA::lux (diamonds) and the parental strains for comparison. Luminescence levels were quantified from liquid cultures and correspond to the mean of three independent experiments.