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. 2023 Jan 18;12(2):200. doi: 10.3390/antibiotics12020200

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Induction of eptA and arn genes in colistin-resistant spontaneous mutants. (a) Box-and-whisker plots showing the luminescence emitted by 160 colistin-resistant spontaneous mutants obtained for each of the PAO1 lux, PAO1 PeptA::lux, and PAO1 Parn::lux strains, on LB agar plates. (b) Example of a screening plate, showing the growth (left image) and luminescence emission (right panel) for a subset (n = 32) of the colistin-resistant mutants of the above-mentioned strains. The parental (colistin-sensitive) strains PAO1 lux, PAO1 PeptA::lux, and PAO1 Parn::lux were included as controls. Asterisks (*) indicate a statistically significant difference (p < 0.001), according to the Kruskal–Wallis test. Abbreviation: ns, not significant.