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. 2023 Jan 24;12(2):261. doi: 10.3390/antiox12020261

Figure 4.

Figure 4

Ferroptosis modulators affect α-Syn aggregation. (A–F) Representative immunofluorescent images were acquired in 1-week-old (A,A’) and 3-week-old (B,B’) untreated α-Syn over-expressing flies, in 1-week-old α-Syn over-expressing flies treated with Erastin (C,C’), in 3-week-old α-Syn over-expressing flies treated with FAC (D,D’), NAC (E,E’) and DEF (F,F’). The red signal corresponds to α-Syn aggregates and the green signal to dopaminergic (DA) neurons. Scale bar 50 μm. (G) Quantitative analysis (mean ± SEM) was performed in 1-week-old untreated αSyn-overexpressing flies and following the treatment with Erastin. The statistical difference was assessed by the Mann–Whitney U test. N: 13 TH > αSyn, 7 Erastin. (H) Quantitative analysis (mean ± SEM) was performed in 3-week-old untreated αSyn-overexpressing flies and following the treatment with FAC, NAC, and DEF. Analysis of variance and Tukey’s multiple comparisons post-hoc test indicate the following statistically significant differences: * TH > αSyn vs. FAC, *** NAC vs. FAC, ** DEF vs. FAC. N: 15 TH > αSyn, 8 NAC, 8 Def, and 7 FAC. ***, **, *, indicate p < 0.001, <0.01, <0.05. For the sake of clarity, only the statistically significant differences between untreated α-Syn over-expressing flies and the other conditions are reported in the graph.