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Membrane disruption of E. coli MG1655 by PMB and LL-37, alone and in combination. Cells of E. coli MG1655 were incubated for 30 min in the presence of MIC concentrations of PMB, LL-37 and PMB + LL-37. Cells were subsequently stained with Syto 9 and propidium iodide for 15 min in the dark. Fluorescence was measured using an ATTUNE NXT Flow Cytometer (Thermo Fisher) until 15,000 events were obtained. Results were analyzed using the Flow Cytometer Software.
