TABLE 1.
Primer | Sequencea | Targetb | Reference |
---|---|---|---|
AN47F | GTTACGCACTGAAATTCCAC | L. lactis hrcA (828) | 4 |
AN48rT7 | TAATACGACTCACTATAGCTACAGGTCCTACAACTG | L. lactis hrcA (1174) | 4 |
AN49F | GCAAAATGTGCAACGTCGCG | L. lactis grpE (1496) | 4 |
AN50rT7 | TAATACGACTCACTATAGCTGTCAGCTGGATGTTCG | L. lactis grpE (1781) | 4 |
AN51F | GCAGTTATCACTGTTCCTGC | L. lactis dnaK (2264) | 4 |
AN52rT7 | TAATACGACTCACTATAGCTTGACCAAGGTCGATTCCG | L. lactis dnaK (2603) | 4 |
AN64 | TGCAAGCACCAATCTTCATC | L. lactis subsp. cremoris 16S rRNA | 16 |
AN65 | ACCCGACAAGGAATTTCGC | Eubacterial 23S rRNA | 1 |
AN66F | TTGGCTTATTGGCAGTTGC | L. lactis dnaK (∼4336) | 4 |
AN67rT7 | TAATACGACTCACTATAGCTGACAGCCCAATTGCCG | L. lactis dnaK (∼4675) | 4 |
AN68R | AGCACTCTTTACCTCTAAG | L. lactis hrcA | 4 |
LLC68Cy3 | Cy3-TGCAAGCACCAATCTTCATC | L. lactis subsp. cremoris 16S rRNA | 16 |
EUB1933 | Cy3-ACCCGACAAGGAATTTCGC | Eubacterial 23S rRNA | 1 |
The nucleotide sequences are shown with the 5′ end to the left. T7 polymerase tail sequences are in bold.
Numbers in parentheses refer to the starting position according to the nucleotide sequence of the dnaK operon (4).