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. 2001 Aug;183(16):4747–4751. doi: 10.1128/JB.183.16.4747-4751.2001

TABLE 1.

Oligonucleotides

Primer Sequencea Targetb Reference
AN47F GTTACGCACTGAAATTCCAC L. lactis hrcA (828) 4
AN48rT7 TAATACGACTCACTATAGCTACAGGTCCTACAACTG L. lactis hrcA (1174) 4
AN49F GCAAAATGTGCAACGTCGCG L. lactis grpE (1496) 4
AN50rT7 TAATACGACTCACTATAGCTGTCAGCTGGATGTTCG L. lactis grpE (1781) 4
AN51F GCAGTTATCACTGTTCCTGC L. lactis dnaK (2264) 4
AN52rT7 TAATACGACTCACTATAGCTTGACCAAGGTCGATTCCG L. lactis dnaK (2603) 4
AN64 TGCAAGCACCAATCTTCATC L. lactis subsp. cremoris 16S rRNA 16
AN65 ACCCGACAAGGAATTTCGC Eubacterial 23S rRNA 1
AN66F TTGGCTTATTGGCAGTTGC L. lactis dnaK (∼4336) 4
AN67rT7 TAATACGACTCACTATAGCTGACAGCCCAATTGCCG L. lactis dnaK (∼4675) 4
AN68R AGCACTCTTTACCTCTAAG L. lactis hrcA 4
LLC68Cy3 Cy3-TGCAAGCACCAATCTTCATC L. lactis subsp. cremoris 16S rRNA 16
EUB1933 Cy3-ACCCGACAAGGAATTTCGC Eubacterial 23S rRNA 1
a

The nucleotide sequences are shown with the 5′ end to the left. T7 polymerase tail sequences are in bold. 

b

Numbers in parentheses refer to the starting position according to the nucleotide sequence of the dnaK operon (4).