Table 3.

Biofilm metabolic activity (%) rates of Staphylococcus aureus and Pseudomonas aeruginosa strains.

Samples	Staphylococcus aureus		Pseudomonas aeruginosa
	ATCC 6538	HT1	ATCC 27853	HT5
Control	100 ± 2.07	100 ± 1.97	100 ± 1.57	100 ± 2.00
EOP
4 mg/mL	58.93 ± 1.19	63.09 ± 5.19	31.56 ± 7.00	43.21 ± 4.96
2 mg/mL	59.57 ± 1.97	70.14 ± 1.96	43.52 ± 3.88	49.23 ± 6.65
1 mg/mL	64.06 ± 3.30	73.22 ± 5.57	57.40 ± 3.62	58.87 ± 3.27
0.5 mg/mL	69.71 ± 3.00	76.59 ± 1.00	67.97 ± 2.78	61.62 ± 6.05
0.1 mg/mL	72.78 ± 1.88	78.97 ± 1.69	79.45 ± 2.35	66.41 ± 4.79
EOPD
4 mg/mL	49.84 ± 2.25	59.06 ± 1.95	34.49 ± 7.04	43.96 ± 7.55
2 mg/mL	59.28 ± 1.35	63.32 ± 6.28	49.25 ± 2.64	50.55 ± 3.03
1 mg/mL	63.83 ± 2.07	69.57 ± 2.88	56.64 ± 3.80	57.92 ± 2.67
0.5 mg/mL	65.76 ± 5.25	73.22 ± 1.21	72.93 ± 2.19	61.59 ± 4.90
0.1 mg/mL	70.67 ± 1.57	76.57 ± 2.16	80.67 ± 1.58	71.80 ± 1.91
Limonene
4 mg/mL	61.78 ± 3.57	58.29 ± 1.82	64.20 ± 3.73	58.56 ± 3.14
2 mg/mL	66.13 ± 3.36	60.70 ± 2.62	68.45 ± 2.66	66.50 ± 2.61
1 mg/mL	72.41 ± 1.94	63.14 ± 1.68	72.13 ± 1.70	71.30 ± 2.00
0.5 mg/mL	74.73 ± 2.16	70.61 ± 4.31	77.54 ± 1.52	78.99 ± 3.53
0.1 mg/mL	83.55 ± 2.14	74.47 ± 4.37	88.23 ± 1.59	84.74 ± 1.70
Ciprofloxacin 5 µg/mL	55.08 ± 2.81	55.16 ± 2.73	19.82 ± 3.12	64.22 ± 0.80

EOP: Essential oil obtained by cold-pressed method; EOPD: essential oil obtained by cold-pressed method followed by steam distillation. Data are presented as means ± SEMs (n = 8) of three independent experiments. All experiments show significant differences compared to respective controls (p < 0.05).