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. 2023 Jan 30;11(2):412. doi: 10.3390/biomedicines11020412

Figure 2.

Figure 2

MYC inhibition by Omomyc and an shRNA against MYC affects RNAPII R1810 symmetrical dimethylation in cancer cells. Cells were treated with doxycycline for 24 h. Thereafter, immunoprecipitation was performed by means of an anti-RNAPII antibody, followed by immunoblotting with anti-RNAPII-R1810me2s, MYC, SMN, and RNAPII antibodies. (a) BT168FO cells. R1810 symmetrical dimethylation was severely impaired in the presence of FlagOmomyc. SMN binding to RNAPII also decreased. (b) BT168 cells infected with a lentivirus encoding a doxycycline-inducible shRNA against MYC. The shRNA strongly decreased RNAPII symmetrical dimethylation and SMN recruitment. (c) RamosFO cells. Also in this cancer cell line, MYC inhibition led to a decrease in RNAPII symmetrical dimethylation and SMN recruitment. Densitometry histograms are shown to the right of each panel. Each bar represents mean ± SEM.