Figure 3.

Autoradiography from 1991 of the first detected TP53 mutations in primary medulloblastoma tissue. Extracted DNA from frozen tumor samples was analyzed by the SSCP-PCR of exon 6 of the TP53 gene incorporating radioactively labelled dCTP. Despite the identical length, conformational changes as a result of point mutations in the sequence compared to wild-type DNA can be revealed on an acrylamide gel when run as single-stranded (denatured) DNA fragments. Of five medulloblastomas (lanes 1–5), two (lanes 3 and 5) show a mutation. DNA from the normal brain served as a control, both denatured (lane 6) and normal (double-stranded, lane 7) (reproduced in part from [1], permitted).