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Overexpression of CCP1 generates ΔC2-α-tubulin more efficiently in cell lines harboring higher levels of ∆Y-α-tubulin. (A,B) Detection of ∆C2-α-tubulin in HeLa, HEK293T, or CHL-1 cells transiently expressing EGFP-CCP1 by immunoblotting of cell lysates (A) and immunofluorescence (B). In merged images in (B), total α-tubulin (DM1A staining) is shown in yellow, EGFP in green, ∆C2-α-tubulin in magenta, and DNA (DAPI staining) in blue. Scale bars in (B), 20 µm.
