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Western blot analysis of SIRT1 (A) and SOD2 (B) and representative images (C). Three mice per experimental group were employed. Experiments were conducted in triplicate. Data obtained from n = 9 observations are presented as means ± SEM of densitometric analysis of immunoreactive bands normalized to internal reference protein (glyceraldehyde-3-phosphate dehydrogenase, GAPDH). * p < 0.01 vs. CTRL, ** p < 0.05, # p < 0.01, t-test.
