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. 2023 Jan 17;13(2):191. doi: 10.3390/biom13020191

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effects of Licos D and E on membrane permeability and [³H]choline uptake in SIM-A9 cells. ATP contents were measured after treatment with 1.5625–100 µM of Licos D (A) and E (B) for 40 min. ATP contents were determined using the CellTiter-Glo^® Luminescent Cell Viability Assay. After preincubation of Licos D (C) and E (D) for 20 min, 10 µM [³H]choline was added and uptake was measured for 20 min. IC₅₀ values of Licos D and E for inhibition of [³H]choline uptake were 17.1 and 12.4 μM, respectively. The results are presented as a percentage of uptake measured with the vehicle control. Values are the mean ± SD (n = 4). ** p < 0.005, *** p < 0.0005 and **** p < 0.0001 compared with vehicle control. Statistical analysis of the data was performed using Dunnett’s multiple comparisons test.

Effects of Licos D and E on membrane permeability and [³H]choline uptake in SIM-A9 cells. ATP contents were measured after treatment with 1.5625–100 µM of Licos D (A) and E (B) for 40 min. ATP contents were determined using the CellTiter-Glo^® Luminescent Cell Viability Assay. After preincubation of Licos D (C) and E (D) for 20 min, 10 µM [³H]choline was added and uptake was measured for 20 min. IC₅₀ values of Licos D and E for inhibition of [³H]choline uptake were 17.1 and 12.4 μM, respectively. The results are presented as a percentage of uptake measured with the vehicle control. Values are the mean ± SD (n = 4). ** p < 0.005, *** p < 0.0005 and **** p < 0.0001 compared with vehicle control. Statistical analysis of the data was performed using Dunnett’s multiple comparisons test.