. 2023 Feb 22;10(2):ENEURO.0340-22.2023. doi: 10.1523/ENEURO.0340-22.2023

Table 1.

Listing of animals used for the five main experiments in the study

Experiment #	Readout	Model	Cellular target	Genotype/AAV	Group	Age (weeks)	Mouse #
1	Vascular structure	DGC-Pten KO	≈20% of dentate granule cells	Gli1-CreER^T2+/−, Pten^wt^/^wt, TdTom^fl^/^wt	Control	4	6 (3 F, 3 M)
				Gli1-CreER^T2+/−, Pten^wt^/^wt, TdTom^fl^/^wt	Control	6	7 (2 F, 5 M)
				Gli1-CreER^T2+/−, Pten^wt^/^wt, TdTom^fl^/^wt	Control	10	6 (3 F, 3 M)
				Gli1-CreER^T2+/−, Pten^fl^/^fl, TdTom^fl^/^wt	KO	4	7 (2 F, 5 M)
				Gli1-CreER^T2+/−, Pten^fl^/^fl, TdTom^fl^/^wt	KO	6	8 (4 F, 4 M)
				Gli1-CreER^T2+/−, Pten^fl^/^fl, TdTom^fl^/^wt	KO	10	11 (5 F,6 M)
2	BBB Integrity	DGC-Pten KO	≈20% of dentate granule cells	Gli1-CreER^T2−/−, Pten^fl^/^fl, tdTom^wt^/^wt	Control	11	3 (3 F, 0 M)
				Gli1-CreER^T2−/−, Pten^fl^/^fl, tdTom^fl^/^wt	Control	11	2 (1 F, 1 M)
				Gli1-CreER^T2+/−, Pten^fl^/^fl, tdTom^wt^/^wt	KO	11	4 (3 F, 1 M)
3	Vascular structure	FB-Pten KO	Forebrain excitatory neurons	CamK2α-Cre^−/−, Pten^fl^/^fl (or Pten^fl^/^wt)	Control	6.5–7	4 (3 F, 1 M)
				CamK2α-Cre^+/−, Pten^wt^/^wt	Control	6.5–7	3 (2 F, 1 M)
				CamK2α-Cre^+/−, Pten^fl^/^fl	KO	6.5–7	5 (3 F, 2 M)
4	Western blotting	FB-Pten KO	Forebrain excitatory neurons	CamK2α-Cre^−/−, Pten^fl^/^fl	Control	7–8	2 (1 F, 1 M)
				CamK2α-Cre^+/−, Pten^wt^/^wt	Control	7–8	5 (3 F, 2 M)
				CamK2α-Cre^+/−, Pten^fl^/^fl	KO	7–8	7 (4 F, 3 M)
5	Vascular structure	f-Tsc2 KO	Focal loss from cortical excitatory neurons	Tsc2^wt^/^wt + AAV9-CaMKII- mCherry-Cre	Control	7, 15, 17, 20	4 (2 F, 2 M)
5	Vascular structure	f-Tsc2 KO	Focal loss from cortical excitatory neurons	Tsc2^fl^/^fl + AAV9-CaMKII- mCherry-Cre	KO	9, 14, 14,15,19	5 (3 F, 2 M)

Experiment #1, DyLight 649 treatment of DGC-Pten KOs and controls to examine vascular structure. Experiment #2, AF488-BSA treatment of DGC-Pten KOs to assess blood-brain barrier permeability. Experiment #3, DyLight 649 treatment of FB-Pten KOs and controls to examine vascular structure. Experiment #4, Western blot analyses of FB-Pten KOs and controls. Experiment #5; CD31 immunostaining of focal Tsc2 KO mice to examine vascular structure.