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. 2023 Jan 27;13(2):238. doi: 10.3390/biom13020238

Figure 2.

Figure 2

Cytotoxic effects of OESA and OESY on HeLa cell line. The inhibitory effects of OESA and OESY on cell growth were determined using a CCK8 assay. Cells were exposed to serial dilutions of OESA and OESY (0.1; 0.2; 0.4; 1; 5; 10; and 20 mg/mL) for 72 h and further incubated with CCK8 tetrazolium salt solution in the dark for 4 h. The absorbance was measured at 460 nm and the % of cell viability was calculated with respect to the untreated cells. Results are expressed as mean ± standard deviation (n = 3). ** p < 0.01, **** p < 0.0001.