Figure 5.

Absolute quantification of viral DNA. HeLa cells and the virus were pre-treated with OESA and OESY at 0.4 and 0.2 mg/mL for 1 h at 37 °C. Then, the pre-treated virus was used to infect pre-treated cells. The infection was carried out at 10 MOI at 37 °C, and 1 h later, the viral inoculum was removed and replaced with growth medium in the presence of both compounds at 0.4 and 0.2 mg/mL. Acyclovir (50 µM) was used as a positive control. The viral DNA was extracted 24 h post-HSV-1 infection as described in the Materials and Methods. Viral DNA amounts were determined via absolute Real-Time PCR using a TaqMan probe. The results are expressed as concentrations in µg of DNA/µL. Statistical analyses were performed in triplicate using a one-way ANOVA analysis assay, and ** p < 0.01 vs. HSV-1 + DMSO indicates significant change.