Figure 7.

Antiviral activity of pure compounds. (A) HeLa cells (4 × 10⁵ cells/wells) and the virus were pre-treated with oleuropein (150 µg/mL, 300 µg/mL, and 400 µg/mL) for 1 h at 37 °C. Then, the pre-treated virus was used to infect pre-treated cells. The infection was carried out at 10 MOI at 37 °C, and 1 h later, the viral inoculum was removed and replaced with growth medium in the presence of both compounds at different concentrations. Acyclovir (50 µM) was used as a positive control. Virus yield was determined at 24 h p.i. using the standard plaque assay on VERO cells. (B) Relative quantification of viral transcripts (ICP0, UL42 and US11) was performed using Real-Time quantitative PCR and analyzed using the comparative Ct method (^ΔΔCt). Values represent ± SD of the average of three independent experiments normalized against GAPDH. (C) Western blot analysis of ICP0, UL42, and Us11 viral proteins. GAPDH was used as a housekeeping gene. Data are expressed as a mean (± SD) of at least three experiments. Statistical analyses were performed using a one-way ANOVA analysis assay. ** p < 0.01, **** p < 0.0001 vs. HSV-1 + DMSO.