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. 2023 Jan 28;12(2):196. doi: 10.3390/biology12020196

Table 2.

Primary and secondary antibodies.

Antigen Immunogen Manufacturer, Species,
Mono-Polyclonal, Cat./Lot.
No., RRID
Dilution
Primary
antibodies
Anti-Interleukin-6
(M-19)
Purified antibody raised against a peptide mapping at the C-terminus of murine IL6 Santa Cruz Biotechnology
(Santa Cruz, CA, USA), Goat polyclonal IgG, Cat# sc-1265, RRID: AB_2127470
1:100
Anti-Transforming Growth Factor β1 (V) Purified antibody raised against a peptide mapping at the C-terminus of TGF-β1
of human origin
Santa Cruz Biotechnology (Santa Cruz, CA, USA), Rabbit polyclonal IgG, Cat# sc-146, RRID: AB_632486 1:100
Anti-Glial fibrillary acidic protein (C-19) Purified antibody raised against a peptide mapping at the C-terminus of GFAP
of human origin
Santa Cruz Biotechnology
(Santa Cruz, CA, USA), Goat polyclonal IgG, Cat# sc-6170,
RRID: AB_641021
1:100
Anti-Nuclear factor erythroid 2–related factor 2 Purified antibody raised against a peptide within Human Nrf2 aa 550 to the C-terminus Abcam (Cambridge, UK),
Rabbit polyclonal IgG, Cat# ab31163,
RRID: AB_881705
1:100
Anti-Superoxide Dismutase-1
(FL-154)
Purified antibody raised against amino acids 1–154 representing full-length human SOD-1 Santa Cruz Biotechnology
(Santa Cruz, CA, USA), Rabbit polyclonal IgG, Cat# sc-11407, RRID: AB_2193779
1:100
Anti-
Cyclooxygenase-2
(M-19)
Purified antibody raised
against a peptide mapping at the C-terminus of COX2
of mouse origin
Santa Cruz Biotechnology
(Santa Cruz, CA, USA), Goat
polyclonal IgG, Cat# sc-1747, RRID: AB_2084976
1:100
Anti-Nitric Oxide
Synthases-2 (M19)
Purified antibody raised
against a peptide mapping at the C-terminus of NOS2
of mouse origin
Santa Cruz Biotechnology
(Santa Cruz, CA, USA), Rabbit polyclonal IgG, Cat# sc-650, RRID: AB_631831
1:100
Anti- N-methyl-D-aspartate Receptors 1 Purified antibody raised
against a peptide corresponding to the C-terminus of rat NMDA receptor subunit
Millipore—Merck KGaA
(Darmstadt, Germany),
Rabbit monoclonal IgG,
Cat# AB9864, RRID: AB_2112158
1:500
Anti-Glutamate Receptor 2 and 3 Purified antibody raised
against a peptide mapping at the C-terminus of rat GluR2
Millipore—Merck KGaA
(Darmstadt, Germany),
Rabbit polyclonal IgG,
Cat# AB1506, RRID: AB_ 90710
1:100
Secondary
Antibodies
Biotinylated goat
anti-rabbit IgG
Gamma
immunoglobulin
Vector Laboratories
(Burlingame, CA, USA),
Goat, lot# PK-6101,
RRID: AB_2336820
1:200
Biotinylated rabbit
anti-goat IgG
Gamma
immunoglobulin
Vector Laboratories
(Burlingame, CA, USA),
Rabbit, Cat# PK-6105, RRID: AB_2336824
1:200