Table 2.
Antigen | Immunogen | Manufacturer, Species, Mono-Polyclonal, Cat./Lot. No., RRID |
Dilution | |
---|---|---|---|---|
Primary
antibodies |
Anti-Interleukin-6 (M-19) |
Purified antibody raised against a peptide mapping at the C-terminus of murine IL6 | Santa Cruz Biotechnology (Santa Cruz, CA, USA), Goat polyclonal IgG, Cat# sc-1265, RRID: AB_2127470 |
1:100 |
Anti-Transforming Growth Factor β1 (V) | Purified antibody raised against a peptide mapping at the C-terminus of TGF-β1 of human origin |
Santa Cruz Biotechnology (Santa Cruz, CA, USA), Rabbit polyclonal IgG, Cat# sc-146, RRID: AB_632486 | 1:100 | |
Anti-Glial fibrillary acidic protein (C-19) | Purified antibody raised against a peptide mapping at the C-terminus of GFAP of human origin |
Santa Cruz Biotechnology (Santa Cruz, CA, USA), Goat polyclonal IgG, Cat# sc-6170, RRID: AB_641021 |
1:100 | |
Anti-Nuclear factor erythroid 2–related factor 2 | Purified antibody raised against a peptide within Human Nrf2 aa 550 to the C-terminus | Abcam (Cambridge, UK), Rabbit polyclonal IgG, Cat# ab31163, RRID: AB_881705 |
1:100 | |
Anti-Superoxide Dismutase-1 (FL-154) |
Purified antibody raised against amino acids 1–154 representing full-length human SOD-1 | Santa Cruz Biotechnology (Santa Cruz, CA, USA), Rabbit polyclonal IgG, Cat# sc-11407, RRID: AB_2193779 |
1:100 | |
Anti- Cyclooxygenase-2 (M-19) |
Purified antibody raised against a peptide mapping at the C-terminus of COX2 of mouse origin |
Santa Cruz Biotechnology (Santa Cruz, CA, USA), Goat polyclonal IgG, Cat# sc-1747, RRID: AB_2084976 |
1:100 | |
Anti-Nitric Oxide Synthases-2 (M19) |
Purified antibody raised against a peptide mapping at the C-terminus of NOS2 of mouse origin |
Santa Cruz Biotechnology (Santa Cruz, CA, USA), Rabbit polyclonal IgG, Cat# sc-650, RRID: AB_631831 |
1:100 | |
Anti- N-methyl-D-aspartate Receptors 1 | Purified antibody raised against a peptide corresponding to the C-terminus of rat NMDA receptor subunit |
Millipore—Merck KGaA (Darmstadt, Germany), Rabbit monoclonal IgG, Cat# AB9864, RRID: AB_2112158 |
1:500 | |
Anti-Glutamate Receptor 2 and 3 | Purified antibody raised against a peptide mapping at the C-terminus of rat GluR2 |
Millipore—Merck KGaA (Darmstadt, Germany), Rabbit polyclonal IgG, Cat# AB1506, RRID: AB_ 90710 |
1:100 | |
Secondary
Antibodies |
Biotinylated goat anti-rabbit IgG |
Gamma immunoglobulin |
Vector Laboratories (Burlingame, CA, USA), Goat, lot# PK-6101, RRID: AB_2336820 |
1:200 |
Biotinylated rabbit anti-goat IgG |
Gamma immunoglobulin |
Vector Laboratories (Burlingame, CA, USA), Rabbit, Cat# PK-6105, RRID: AB_2336824 |
1:200 |