FIG. 2.
Purification of recombinant His-ΔMGT. An aliquot containing His-ΔMGT was loaded onto a 4-to-20% gradient gel for separation, followed by Coomassie blue staining. Lane 1, whole-cell lysate; lane 2, solubilized fraction; lane 3, Ni2+ column pools; lanes 4 and 5, monomer and oligomer pools after Superdex-75 column chromatography, respectively. Lane 6 reflects the results of the Western blot analysis of purified His-ΔMGT (50 ng) using the polyclonal anti-His-ΔMGT antibodies as described in Materials and Methods.