FIG. 3.

Self-interaction of S. aureus His-ΔMGT. (A) Gel filtration analysis of His-ΔMGT. Shown are the elution profiles of purified His-ΔMGT proteins from a Superdex-75 column in the absence (dashed and dotted line) and presence (dotted line) of 0.5% CHAPS detergent. Elution positions for protein standards are marked. (B) Chemical cross-linking of purified His-ΔMGT. Purified His-ΔMGT (5 μg) was incubated with either the solvent dimethyl sulfonate (lane 1) or the linker disuccinimidyl suberate (lane 2) for 2 h at 4°C as described in the text. The samples were then subjected to separation by SDS-PAGE followed by Coomassie blue staining. Cross-linked complexes are indicated by arrows.