Table 1.

Proportions of developmental deformities scored after 24, 48, 72, 96, and 144 h of thiram exposure.

Time	Deformity (%)	DMZF (DMSO)	LTZF (0.4 µg/L Thiram)	HTZF (4.0 µg/L Thiram)
24 hpf	Edema symptoms	1.71 ± 1.48	1.71 ± 2.96	12.80 ± 5.47
	Abnormal somites	1.66 ± 1.44	3.40 ± 1.50	50.83 ± 6.29 *
48 hpf	Low retina pigment	3.42 ± 1.59	2.59 ± 2.56	31.84 ± 8.07 *
	Abnormal tail blood flow	3.42 ± 1.59	1.71 ± 2.96	22.16 ± 4.45 *
72 hpf	Unhatched embryos	0.88 ± 1.52	3.47 ± 1.44	99.05 ± 1.65 *
	Pericardial edema	1.69 ± 1.46	6.08 ± 1.41	32.20 ± 6.38 *
	Yolk sac edema	0	2.56 ± 2.56	37.10 ± 5.49 *
96 hpf	Unhatched embryos	0.88 ± 1.52	1.78 ± 1.54	99.02 ± 1.70 *
144 hpf	Body curvature	0	0	100.00 *
	Wavy distortions of notochord	0	0	99.02 ± 1.70 *
	Abnormal touch response	0	0	46.10 ± 6.95 *

Data are shown as the mean ± standard deviation of three independent experiments (40 embryos in each experiment (body curvature, wavy distortions of notochord, and abnormal touch response 10 embryos in each experiment)). Unpaired Student’s t-test was performed for assessing statistical significance. * Statistically significant (p < 0.05).