Figure 2.

Clarithromycin inhibits CSF-mediated GABA receptor potentiation and activation. Whole cell voltage clamp recordings from HEK293 cells expressing α1β2γ2s GABA_ARs. (a) CSF from a patient diagnosed with Idiopathic Hypersomnia reversibly enhances/potentiates currents activated by 10 μM GABA (EC₁₀). The potentiation is reduced in the presence of 300 μM clarithromycin. (b) Clarithromycin has no intrinsic efficacy at GABA_A receptor, but reversibly inhibits receptor activation. Bars above the current traces indicate the duration of agonist/modulator application. Calibration bars indicate current amplitude and duration in pA and seconds.