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Competition for binding to a UGA PTC between release factor complex, leading to peptidyl-tRNA hydrolysis and nascent peptide release (i.e., termination), and aminoacyl suppressor tRNA ternary complex, leading to readthrough. In our standard HT assay, Stop-POST5 contains FK*VRQ-tRNA in the P-site, where K* denotes a Lys residue labeled with Atto647 on the ε-amino group.
