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. 2023 Jan 30;13(2):255. doi: 10.3390/biom13020255

Figure 1.

Figure 1

Phase-contrast microscopy images of control (a) and hypertrophic (b) 3T3-L1 cells on day 12 of adipogenic differentiation (100× magnification). Hypertrophy was induced by addition of palmitic acid (PA, 0.5 mM) to DMEM (+10% FBS) culture medium. 3T3-L1 cells were first subjected to differentiation in DMEM (+10% FBS) culture medium supplemented with 3-isobutyl-1-methylxanthine (IBMX, 0.5 mM), dexamethasone (DEX, 1 μM), insulin (0.5 μM), and rosiglitazone (2 μM) for 3 days, followed by 3 day culture in DMEM (+10% FBS) culture medium comprising insulin (0.5 μM) and rosiglitazone (2 μM). PA (0.5 mM) was administered to 3T3-L1 adipocytes from day 8 to day 12 of differentiation. Enlarged lipid droplets in adipocytes presented on image (b) are a characteristic feature of hypertrophy.