Figure 2.

Effect of co-treatment with PF and hyperthermia on AGS cell viability. AGS cells were incubated for 24 h with PF (0, 100, and 200 μg/mL) with or without hyperthermia at 43 °C. (a) The MTT assay was used to calculate the percentage of cell viability, and Compusyn software was used to calculate the combination index. (b) Trypan blue assay. (c) Morphological variations indicating apoptosis were observed using a microscope. (d) Crystal violet staining was used for the clonogenic assay. (e) Wound healing assay. * p < 0.05, ** p < 0.01, *** p < 0.001 vs. control group; ## p < 0.01, ### p < 0.001 vs. 43 °C + 0 μg/mL group by Student’s t-test. PF, Ponciri Fructus Immaturus.