Figure 3.

Effects of HMOS on IL13-mediated IEC barrier disruption. T84 cells were grown on transwells and (A) exposed to the Th2-type cytokines IL4, IL5, IL9 and IL13 after which transepithelial electrical resistance (TEER) was measured at 24 h and 48 h. To investigate preventive effects of HMOS on barrier disruption by IL13, IEC were preincubated with (B) 2′FL and 3FL, (C) 3′SL and 6′SL, and (D) LNnT 24 h prior to exposure to IL13. Data is analyzed by Two-Way ANOVA followed by a Bonferroni post-hoc test, n = 6, mean ± SEM (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001).