TABLE 1.

Bacterial strains and plasmids

Bacterial strain or plasmid	Description	Source or reference
R. equi
ATCC 6939	Type strain	30
MAD	Cholesterol oxidase-hyperproducing strain	42
103+	Clinical isolate with virulence plasmid	8
103−	103 derivative lacking virulence plasmid	8
RHE3-15+	choE knockout mutant of 103+	This work
RHE3-19	choE knockout mutant of 103−	This work
L. ivanovii
ATCC 19119	Type strain	Collection
E. coli
DH5α	Cloning host strain	Our laboratory
HB101	Cloning host strain	Our laboratory
TG1	Cloning host strain	Our laboratory
Plasmids
pUC19	Cloning vector	54
pGEM-Te	T-vector for cloning of PCR products	Promega
pPE207	E. coli-Mycobacterium shuttle vector containing apramycin resistance marker	37
pVK173-T	E. coli-Mycobacterium shuttle vector containing hygromycin resistance marker	37
pRE7	E. coli-R. equi shuttle vector	55
pRHE1	pUC19 inserted with choE	This work
pRHE2	pUC19 inserted with accC4	This work
pRHE3	pUC19 with choE::accC4 mutant allele (suicide vector for choE mutagenesis by gene replacement)	This work
pRHE4	pGEM-Te inserted with choE and its natural promoter	This work
pRHE5	pVK173-T inserted with choE and its natural promoter	This work
pRHE6A	pRE7 inserted with accC4	This work