TABLE 3.
Recombination frequencies of the suicide plasmid pRHE3 (containing choE target sequences) or pRHE2 (no choE target sequences) in R. equi
| Suicide plasmid | Recombination frequencya in strain:
|
|
|---|---|---|
| 103+ | 103− | |
| pRHE3 | 1.9 × 10−3 | 7.5 × 10−3 |
| pRHE3ss | 11.5 × 10−3 | 47.5 × 10−3 |
| pRHE2 | <10−5 | <10−6 |
| pRHE2ss | <10−5 | <10−6 |
a
Recombination frequencies were calculated as the ratio between transformation frequencies (expressed as Aprr colonies per microgram of DNA) obtained with the suicide plasmids and with the pRHE6A vector, which replicates in R. equi.