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Morphological changes observed in MCF10A cells after prolonged, chronic exposure to NO containing. MCF10A cells were plated at a density of 4 × 105 per 60 mm dish and were treated with 0 (A), 100 (B), 300 (C), or 500 (D) μM DETANO twice weekly for three weeks, and then the DETANO was removed, and the cells were analyzed. Elongated cells (black arrow) were observed in NO treated cells.
