Figure 2.

Scratch Test. NO treatment was withdrawn and MCF10A cells were seeded in 60 mm dish and grown to confluence in complete media without treatment. Upon reaching confluence, a straight line was scratched across each confluent monolayer using a 1000 μL pipette tip and the cells were allowed to close the gap in the absence of NO treatment. Top panels show the Control (left) as well as cells that had been maintained in 100 μM DETANO (right) treated MCF10A cells at time 0. The bottom panels show the same cells at 96 h; when compared to the untreated control, MCF10A cells that had been maintained in 100 μM DETANO closed the gap faster.