Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Feb 7;13(2):311. doi: 10.3390/biom13020311

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Anchorage Independent Growth in Soft Agar. (A) 6-well cell culture plates were prepared with a bottom layer consisting of 0.5% agarose in SIT medium with 5% FBS added. The top layer included 50,000 cells suspended in 3 mL of 0.3% agarose in SIT medium. Black arrows show an acini-like spheroid with hollow lumen formed by control MCF10A cells. White arrows show a clustered and disorganized spheroid formed by MCF10A cells previously maintained in 100 μM DETANO. (B) * p < 0.05 significantly increased colony formation in soft agar, when compared to untreated control.