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. 2023 Feb 20;11(2):635. doi: 10.3390/biomedicines11020635

Figure 4.

Figure 4

Inhibition of FOSL1 can attenuate VSMC calcification through upregulation of SLC7A11 and inhibition of ferroptosis. (A) Mineral deposition in VSMCs was detected by ARS staining and (B) calcium content assay; n = 5 per group. (C) P53, SLC7A11, GSS, and FSP1 mRNA expression were determined by RT-qPCR; n = 6 per group. (D) Western blot analysis of FOSL1, P53, SLC7A11, SP7, and MSX2 protein expression; n = 3 per group. (E) Cellular GSH levels were measured as described in the Section 2; n = 3 per group. Statistical analysis was performed using one-way ANOVA (Tukey honestly significant difference post hoc test) * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.