Figure 4.

Analysis of total GTP-DRP1 protein levels and total DRP1 post-translational modification protein levels at serine 616 and serine 637 post single mechanical exposure—in vitro model of bTBI. Western blotting was conducted using an automatic capillary-based system (Wes, Protein Simple) to look at relative protein quantification to identify possible fission post-overpressure exposure. Only for qualitative visual comparison, digitized bands illustrate how proteins moved through the capillary system on the Wes. (a) There were no significant changes in DRP1 protein levels at 4 h, 24 h, and three days following the mechanical insult. (b) The total levels of p-DRP1^s616 protein were increased by four hours and returned to physiological levels by three days post single mechanical exposure. (c) The total levels of p-DRP1^s637 protein were maintained at physiological levels at all-time points post single mechanical exposure (n = 6/group; data are mean ± SEM; * p-value represents ≤ 0.05; ns-value represents not significant).