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. 2023 Jan 23;13(2):219. doi: 10.3390/biom13020219

Figure 3.

Figure 3

Chemical chaperone and antioxidant activities of ZN/VB compounds. (A) Thioflavin T binding assay for TauRD (Q244-E372 of Tau, NP_005901) aggregation. Top: Amino acid sequence of TauRD-His protein, with TauRD marked in red, ΔK280 marked in purple, in-frame-fused His6 marked in pink, and N-terminal Met and linker (Leu and Glu) marked in blue. Bottom: Anti-aggregation assay. TauRD protein (20 μM) was incubated with congo red, ZN-013, ZN-015, VB-030 or VB-037 (2.5–20 μM) at 37°C for 48 h, and aggregation was monitored by measuring thioflavin T fluorescence intensity (n = 3). p values: comparisons between 0 h and 48 h (###: p < 0.001), wild type versus ΔK280 (&&&: p < 0.001), or with and without compound addition (*: p < 0.05, **: p < 0.01, ***: p < 0.001) (one-way ANOVA with a post hoc Tukey test). (B) Antioxidant assays of kaempferol (as a positive control) and ZN/VB compounds (10–160 μM for DPPH free radical scavenging activity and 4–100 μM for trolox equivalent oxygen radical absorbance capacity) (n = 3).