Figure 4.
Neuroprotective effects of ZN/VB compounds in ΔK280 TauRD-DsRed SH-SY5Y cells. (A) High-content assessment of neurite outgrowth (TUBB3 stain, green) (n = 3). The relative length, process, and branch of neurite of uninduced cells was normalized (100%). Nuclei were counterstained with DAPI (blue). The multi-colored mask was applied on segmented fluorescence images to assign the outgrowth of a cell body for quantification. Different colors outline different neurons and their respective neurite outgrowth. The arrows indicated in the uninduced photo are process (red) and branch (white). The total length, process and branch of neurite were calculated. (B) Caspase-1, caspase-6, and (C) AChE activity assessments (n = 3). The relative caspase-1/caspase-6/AChE activity of uninduced cells (Dox-) was normalized (100%). p values: comparisons between induced (Dox+) vs. uninduced (Dox-) cells (#: p < 0.05), or compound-treated vs. untreated (induced) cells (*: p < 0.05, **: p < 0.01, ***: p < 0.001) (one-way ANOVA with a post hoc Tukey test).