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. 2023 Feb 11;12(4):582. doi: 10.3390/cells12040582

Figure 3.

Figure 3

Degradation of cardiac mitochondria is required for MSC activation. (A) Transmission electron micrographs taken 24 h after exposure of MSCs to cardiac mitochondria (right panel, Mito 3 concentration; left panel, non-treated MSCs). Red arrows: intact mitochondria. White arrows: autophagolysosomes. Scale bar: 1 µm. (B) Mtphagy dye fluorescence intensity in cardiac mitochondria-preconditioned MSCs in reference to untreated ones (n = 10). (C–I) Prior cardiac mitochondria transfer at the Mito 3 concentration, MSCs were treated or not with chloroquine (Chloro) and compared with their respective controls. (C–F) Relative mRNA levels of (C) Ki67 (n = 10); (D) VEGF (n = 6) and HGF (n =10); (E) CXCL1, CXCL5, CXCL6, IL11, IL33 and LIF (n ≥ 8); (F) MMP1, MMP9 and MMP14 (n ≥ 7). (G,H) Relative protein secretion levels of (G) VEGF (n = 8) and HGF (n = 5) and (H) CXCL1, CXCL5, CXCL6, IL11, IL33 and LIF (n = 5). (I) Relative collagenase activity (n = 6). Unpaired Student’ t-test in (B). One-way ANOVA with Tukey’s multiple comparisons test in (C–I). * p < 0.05, ** p < 0.01, *** p < 0.001. Each dot represents an independent experiment. Bar graphs represent mean values ± SD.