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. 2023 Feb 7;12(4):533. doi: 10.3390/cells12040533

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© 2023 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Intermittent fasting increased biliary lipid secretion in Apoe^-/- mice fed CD. The gallbladder was cannulated, and bile was collected for 30 min, after a stabilization time of 30 min. Biliary secretions of cholesterol (A), bile acids (B) and phospholipids (C) were determined in ad libitum (AL) or intermittent fasting (IF) Apoe^-/- mice fed chow-diet (CD) or high-fat high-cholesterol diet (HFCD). Values are expressed as means ± SEM (n = 6–7 per group); ns, not significant; ** p < 0.01, *** p < 0.001.