FIG. 3.

BfpB and BfpG coimmunoprecipitate. (A) An antiserum to BfpG coimmunoprecipitates BfpB. Immunoprecipitates were heated to 80°C in SDS-PAGE sample buffer, separated by PAGE, transferred to a nylon membrane for Western analysis, and probed with anti-BfpB antiserum. Lane 1, B171-8ΔG immunoprecipitated with anti-BfpG; lane 2, B171-8 immunoprecipitated with anti-BfpG; lane 3, B171-8ΔB immunoprecipitated with anti-BfpB; lane 4, B171-8 immunoprecipitated with anti-BfpB. Both the anti-BfpB and the anti-BfpG antibodies were able to immunoprecipitate BfpB protein. The dark abundant bands between BfpB and BfpG are rabbit immunoglobulins, Western reactive with the HRP-labeled secondary donkey anti-rabbit immunoglobulin G antibody used in Western detection. (B) An antiserum to BfpB coimmunoprecipitates BfpG. The membrane shown in panel A was sequentially probed with anti-BfpG antiserum without prior stripping of the BfpB antibodies. Both the anti-BfpB and anti-BfpG antibodies were able to immunoprecipitate BfpG protein.