FIG. 5.

BfpB requires BfpG for multimerization but not localization to the outer membrane. Three micrograms of outer- and inner-membrane proteins from B171-8 and B171-8ΔG was loaded onto a 15% polyacrylamide gel. Western blot analysis with anti-BfpB antiserum was performed. Samples on the left half were incubated at 100°C in sample buffer for 7 min, while samples on the right were incubated at 22°C for 7 min.