FIG. 6.

BfpB forms a HMW complex in the absence of BfpA expression. Cells were collected from cultures of either the wild-type strain B171-8 or the BfpA knockout mutant, B171-8ΔAcm, at the indicated times and sonicated, and equal amounts of protein were loaded onto 12% polyarcylamide gels. Western analysis with anti-BfpB antiserum was performed with B171-8 (left) and B171-8ΔAcm (right). Arrowheads, BfpB monomers; stars, BfpB complexes.