FIG. 8.

Comparative analysis of proteins accumulating in tissue culture medium during growth of the wild-type strain, B171-8, and the BfpB deletion strain, B171-8ΔB. Tissue culture medium was collected 3, 5, and 8 h after a 1:100 dilution of an overnight culture into DMEM, and the cells were pelleted by centrifugation. The supernatants were filter sterilized, concentrated 300-fold by ultrafiltration, and analyzed by SDS-PAGE and Coomassie blue staining. In the absence of the BfpB HMW complex, the secretion of proteins into the media is reduced at the 8-h time point. Each lane contains 25 μl of concentrated supernatant from either B171-8 or B171-8ΔB culture media collected at the indicated time point.