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. 2023 Feb 8;13(2):286. doi: 10.3390/brainsci13020286

Figure 7.

Figure 7

AQP4 IF intensity changes in the BS following double blast exposure. AQP4 in control (Ctl.) versus double blast (2×B) rat BS. (A) AQP4-ir positive staining in the BS of a control rat not exposed to blast versus BS of a rat exposed to double blast, taken at 10× magnification. The insets at 40× magnification, defined as superior raphe obscurus-mfl, indicate the area (dorso-medial area) of MFI quantification. (B) Graphs showing MFI of total BS, 50% superior BS midline, and dorso-medial area including the superior portion of the raphe obscurus nuclei and mlf. * indicates p values < 0.05 and ** indicates p values < 0.01 as determined by 2-tailed, unpaired t-tests. Indices are MFI of each ROI over MFI of the total brainstem. IF = Immunofluorescence; BS = brainstem; mlf = medial longitudinal fasciculus; indices = IF intensity ratios between total BS/dorso-medial areas; MFI = mean fluorescent intensity.