Figure 2.

Mebendazole decreases the transcriptional activity of HIFs. (a,b) MDA-MB-231 cells were transfected with either the pGL4.32-GW-LDHA reporter plasmid (a) or the p2.1 reporter plasmid (b). The cells were exposed to 20% O₂ (normoxic) or 1% O₂ (hypoxic) conditions followed by 48 h of treatment with MBZ (0.5 μM and 1 μM) or DMSO as vehicle control. Firefly fluorescence was normalized to Renilla luciferase luminescence reporter assay. Mean ± SEM; n = 2 independent experiments with n = 4 technical replicates. p-values refer to one-way ANOVA test ** < 0.01 or **** < 0.0001.