Figure 4.

Mebendazole decreases HIF-inducible gene products in a dose-dependent manner. (a–f) Relative expression of NDRG1, CA9, and EPO measured by real-time-qPCRs of cDNA in MDA-MB-231 (a–c) and SUM159 (d–f) cells treated for 48 h with MBZ (0.25 μM, 0.5 μM, and 1 μM) or DMSO (vehicle control) exposed to 20% O₂ or 1% O₂. Mean ± SEM; n = 3 independent experiments and n = 3 technical replicates. p-values refer to one-way ANOVA test ** < 0.01,*** < 0.001,**** < 0.0001. (g,h) NDRG1 and CA9 protein expression were analyzed with an immunoblot assay using lysate extracted from MDA-MB-231 (g) and SUM159 (h) cell lines treated with increasing doses of MBZ as indicated. See Supplementary S1 for uncropped blots for (g,h).