Figure 5.

Loss of DA neurons in fPD-hMBOs. Representative images from healthy hMBOs and fPD-hMBOs, immunostained for TH (green) and MAP2 (red), at 120 DIV (A) and 180 DIV (B). Nuclei were stained with DAPI. Scale bar is 25 µm. (C). Quantification of TH immunostaining at 120 and 180 DIV. Values represent mean +/− standard error of mean (SEM) from n = 3 hMBOs per group. Three sections were analyzed from each hMBO. The results were analyzed using two-way ANOVA [SNCA copy number: F (1, 8) = 29.15, p = 0.0006; time: F (1, 8) = 0.4270, p = 0.5318; SNCA copy number × time: F (1, 8) = 0.001276, p = 0.9724)] followed by the Tukey post hoc multiple comparisons test. * p < 0.05.