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. 2023 Feb 16;15(4):1261. doi: 10.3390/cancers15041261

Figure 8.

Figure 8

LINC01094 promotes migration of tumor cells through the PTEN/AKT pathway by targeting AZGP1 in GC. (A) The western blot results showed that only AZGP1 specifically combined LINC01094. (B) The qRT-PCR results showed that LINC01094 was significantly more enriched with the anti-AZGP1 antibody than with the rabbit IgG. (C) The qRT-PCR results showed that the RNA level of PTEN underwent no significant change after overexpression of AZGP1 in HGC-27 and MGC-803 cells. (D) Western blotting was applied to assess the expression levels of PTEN, pAKT, and total AKT after overexpressing AZGP1 in HGC-27 and MGC-803 cells. (E) The qRT-PCR results showed that the RNA level of PTEN underwent no significant change after overexpression of LINC01094 in HGC-27 and MGC-803 cells. (F) Western blotting was applied to assess the expression levels of AZGP1, PTEN, pAKT, and total AKT after overexpressing LINC01094 in HGC-27 and MGC-803 cells. (G) Rescue experiments showed that the migration-promotion effect caused by overexpression of LINC01094 could be rescued by overexpression of AZGP1 (objective: 10×). (H) The western blot results showed that the downregulation of PTEN and upregulation of pAKT effects caused by overexpression of LINC01094 could be rescued by overexpression of AZGP1. (I) Rescue experiments showed that the migration-promotion effect caused by overexpression of LINC01094 could be rescued by perifosine (objective: 10×). (J) The western blot results showed that perifosine successfully downregulated the expression of pAKT. *: p < 0.01, **: p < 0.01, ***: p < 0.001, ns: no statistical significance. The scale bar equals 100 μm, as indicated in each graph.